CLSM, 30 optical sections, reconstruction, NIH Image 1.62.

CLSM and 3D imaging of a stomata on the epidermal surface of a canned green bean. The image shown is a typical "projection"  image comprised of 38 separate optical planes. This is a 200 µm  vibratome cross-section of the bean with the epidermal surface oriented en face  to reveal the depth of the section and the stomata of interest.